Journal: Oncology reports

Article Title: Nogo-B promotes the epithelial-mesenchymal transition in HeLa cervical cancer cells via Fibulin-5.

doi: 10.3892/or.2012.2069

Figure Lengend Snippet: Figure 2. Nogo-B functions as a tumor suppressor gene in HeLa cells. (A) The distribution of endogenous Nogo-B in HeLa cells. HeLa cells were stained with a goat anti-Nogo-B antibody followed by a FITC anti-goat antibody (green) to visualize the localization of Nogo-B. The localization of endogenous Nogo-B was clearly observed in the ER and on the plasma membrane. (B) The components of the plasma membrane (PM) and endoplasmic reticulum (ER) were extracted from HeLa cells, and equivalent proteins were detected by western blotting. The results showed that Nogo-B was expressed mainly in the ER but also on the PM. (C) Western blotting of Nogo-B expression in Blank and different stably expressing Nogo-B cell lines. (D) The proliferation curves of Blank, NB-2 and NB-4 cells, which show that Nogo-B suppresses HeLa cell proliferation.

Article Snippet: Anti-Nogo-B, anti-Fibulin-5 and antiβ-actin antibodies, horseradish peroxidase-conjugated anti-goat and anti-rabbit IgG antibodies, Cy3-conjugated goat anti-rabbit IgG antibodies and FITC-conjugated rabbit anti-goat IgG antibodies were purchased from Santa Cruz Biotechnology, Inc. Anti-E-cadherin, anti-N-cadherin and anti-vimentin antibodies were purchased from Cell Signaling Technology.

Techniques: Staining, Clinical Proteomics, Membrane, Western Blot, Expressing, Stable Transfection